Hoebe group – Advanced Fluorescence Microscopy Development

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Contact: r.a.hoebe@amc.uva.nl

Research overview

Developments in Advanced Fluorescence Microscopy

Super-resolution: blabla

Imaging Living cells: blabla

SPAD Imagers for Super-Resolution Microscopy

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Rescan Confocal Microscopy

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Spatial Controlled Illumination Microscopy

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Figure 3. Confocal fluorescence image sequence of HeLa cells with centromeres labelled with GFP combined with phase contrast. Left without and right with SCIM.

People

PhDs
Ivan Michel Antolovic (2018)
Venkat Krishnaswami (2017)
Giulia De Luca (2016)

Students
Emilie Desclos (2017)
Nathalia Langendijk (2014)


Collaborations
Erik Manders (UvA)
Edoardo Charbon (TUDelft)
Kees Jalink (NKI)
Hans van der Voort (SVI)

 

Publications

Key publications

Antolovic, I. M., S. Burri, C. Bruschini, R. Hoebe and E. Charbon (2016). Analyzing blinking effects in super resolution localization microscopy with single-photon SPAD imagers. Single Molecule Spectroscopy and Superresolution Imaging IX, International Society for Optics and Photonics.

Antolovic, I. M., S. Burri, C. Bruschini, R. A. Hoebe and E. Charbon (2017). “SPAD imagers for super resolution localization microscopy enable analysis of fast fluorophore blinking.” Scientific reports 7: 44108.

Antolovic, I. M., S. Burri, R. A. Hoebe, Y. Maruyama, C. Bruschini and E. Charbon (2016). “Photon-counting arrays for time-resolved imaging.” Sensors 16(7): 1005.

De Luca, G., R. Breedijk, R. Hoebe, S. Stallinga and E. Manders (2017). “Re-scan confocal microscopy (RCM) improves the resolution of confocal microscopy and increases the sensitivity.” Methods and applications in fluorescence 5(1): 015002.

De Luca, G. M., R. M. Breedijk, R. A. Brandt, C. H. Zeelenberg, B. E. de Jong, W. Timmermans, L. N. Azar, R. A. Hoebe, S. Stallinga and E. M. Manders (2013). “Re-scan confocal microscopy: scanning twice for better resolution.” Biomedical optics express 4(11): 2644-2656.

De Vos, W. H., R. A. Hoebe, G. H. Joss, W. Haffmans, S. Baatout, P. Van Oostveldt and E. M. Manders (2009). “Controlled light exposure microscopy reveals dynamic telomere microterritories throughout the cell cycle.” Cytometry Part A 75(5): 428-439.

Hoebe, R., H. Van Der Voort, J. Stap, C. Van Noorden and E. Manders (2008). “Quantitative determination of the reduction of phototoxicity and photobleaching by controlled light exposure microscopy.” Journal of microscopy 231(1): 9-20.

Hoebe, R., C. Van Noorden and E. Manders (2010). “Noise effects and filtering in controlled light exposure microscopy.” Journal of microscopy 240(3): 197-206.

Hoebe, R., C. Van Oven, T. Gadella Jr, P. Dhonukshe, C. Van Noorden and E. Manders (2007). “Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging.” Nature biotechnology 25(2): 249.

Krishnaswami, V., G. M. De Luca, R. M. Breedijk, C. J. Van Noorden, E. M. Manders and R. A. Hoebe (2017). Spatially-controlled illumination with rescan confocal microscopy enhances image quality, resolution and reduces photodamage. Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, International Society for Optics and Photonics.

Krishnaswami, V., C. J. Van Noorden, E. M. Manders and R. A. Hoebe (2014). “Towards digital photon counting cameras for single-molecule optical nanoscopy.” Optical Nanoscopy 3(1): 1.

Krishnaswami, V., C. J. Van Noorden, E. M. Manders and R. A. Hoebe (2016). “Spatially-controlled illumination microscopy: For prolonged live-cell and live-tissue imaging with extended dynamic range.” Quarterly Reviews of Biophysics 49.

More publications:  scholar.google

 

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